| 1. | After one - step reverse transcription of the rna virus genomes , the viruses " specific dna fragments were amplified by nested three loci pcr , which the amplification system was optimized by uniform design
选择合适的核酸抽提方法，一步法抽提hbv 、 hcv及hiv 1的病毒核酸并一步法逆转录病毒的rna ，获得病毒的dna 。 |
| 2. | However , a surprising finding of the present study was the isolation of a sars - coronavirus sequence that had a further deletion of 386 units of the genetic code in the same region of the virus genome
令人感到意外的是，疫症后期在人类身上发现的sars冠状病毒基因组列序，该特定的部位上发现缺少了多达386个基因密码。 |
| 3. | For diagnostic tests to be effective for the detection of the different strains of the sars - coronaviruses , the tests should be developed to target regions of the virus genome that are less likely to mutate
鉴于sars冠状病毒有不同的种类，在研制测试的过程中，必须针对病毒基因组列序中比较稳定的部份，才能有效地测试sars病毒。 |
| 4. | Two plasmids , which contain hbv specific dna fragment and hsv1 dna fragment , were amplified by solid phase two loci pcr and detected by enzymatic indicator system on a gene chip that was constructed by primer immobilization and modified with thiol group on chip surface . for building detection technology by dna chip in clinical , the virus genomes were extracted from the clinical positive samples by one - step nucleic acid extraction
采用已建立的芯片制备方法，在该六种质粒中选择含有hbv与hsv1两种病毒dna序列的质粒为模板，进行同相两重pcr ，采用芯片酶学枪测对固相两重pcr的扩增结果进行检测，得到良好的芯片酶学检测结果。 |
| 5. | The ns5 protein of dengue virus is the largest molecule encoded by the virus genome with a molecule weight of 104 000 . recent research work indicates that the ns5 protein acts as the rna - dependent rna polymerase ( rdrp ) in virus which has the ability to recognize and bind its template rna to synthesize a complementary strand
近年来的研究表明， ns5蛋白具有rna依赖的rna聚合酶( rdrp )功能，即可以识别具有相对特异性的模板rna并与之结合，合成与模板互补的rna链，在病毒基因组复制过程中起关键作用。 |
| 6. | The highest rate of mutation is seen in the part of the virus genome coding for the spike protein , the component of the virus which enables human cell entry . these data suggest that during the early part of the epidemic , the sars - coronavirus was under significant pressure to change in order to become an efficient human virus
这证明来自动物的病毒在感染人类的初期，病毒受到强大的选择压力，而须迅速变异，使病毒得以在人类身上适应和生存，令人类受到感染和传播病毒。 |
| 7. | Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain , and further study and utilize mutation , deletion , insertion and substitution of csfv gene on dna molecular level
中国猪瘟兔化弱毒(脾淋毒)全长感染性cdna的克隆和构建，可以使我们得到纯粹的csfvc -株rna病毒基因组，在dna水平上研究和利用csfv的基因突变、缺失、插入和替换。 |
